A Truly, Walkaway Solution for End-to-End Library Construction

Designed with Next Generation Sequencing (NGS) in mind, the Sciclone® G3 NGSx iQ™ workstation provides unattended automation of even the most complex NGS library construction workflows. The thoughtful collection of high capacity tip storage, on-deck thermal accessories, and highly reproducible liquid transfer technology assures the construction of high-quality NGS libraries from DNA or RNA. The library of validated methods for NGS library construction ensures rapid set up.

NGS sample preparation is a bottleneck that can prevent optimal use of sequencer capacity. The Sciclone® G3 NGSx iQ™ workstation offers walk-away library preparation with consistent and reliable results. The Sciclone® G3 NGSx iQ™ workstation reduces the library preparation bottleneck by implementing validated protocols on an integrated benchtop liquid handler and robotic microplate handler with a large tip and plate capacity. The workstation has 23 deck locators to accommodate all consumables, 5 of which are accessible by the Twister® III Microplate and Tip Handler for automated tip drop-off and removal of spent tip racks, plates, and lids. A gripper mounted to the pipetting arm of the Sciclone® G3 NGSx iQ™ workstation facilitates the transfer of plates, tip boxes, and lids between the 23 deck locators. The Sciclone® G3 NGSx iQ™ workstation includes three 4-110°C temperature-controlled locators, one 4-70°C shaking locator, and an on-deck thermal cycler as well as the other hardware and software required for the methods. Tips and tip boxes are disposed through separate chutes separating contaminated from non-contamintated plastic, reducing hazardous waste disposal costs.

The ever-increasing demand for NGS requires faster, easier, and more accurate library construction. To meet this demand PerkinElmer has created the new Sciclone® NGS iQ™ workstation, a fully-automated, walk-away, easy to use, and accurate library preparation system.The increased degree of automation is obtained through the incorporation of an on deck thermal cycler (ODTC), a robotic arm, increased storage capacity, intuitive graphical user interface, and a continually growing library of PerkinElmer expertly validated NGS automation methods from a wide range of vendors into the Sciclone® G3 NGSx iQ™ workstation, allowing you to walk-away from your instrument and focus on intellectual contributions such as data analysis or designing your next experiment.

The Sciclone® NGSx iQ™ workstation is the instrument of choice for NGS library creation.

Experience Push-Button NGS Library Construction

The Sciclone® G3 NGSx iQ™ workstation has been designed for customer ease of use. Our team of application specialists have created a library of push-button library construction protocols for the most common NGS methods along with a simple graphical user interface that allows you to run a protocol within minutes of installation.

Catering to a broad range of users’ expertise, the interface has been designed to enable full protocol control with a few simple clicks or to give access to sub routines within the protocol enabling error recovery and rapid access to sub-methods to modify sample inputs. Customize any validated method to your specific requirements or create methods from scratch for customer workflows.

Increase Your Throughput with Robotic Tip Storage

With integrated tip storage and a tip transfer provision, the Sciclone® G3 NGSx iQ™ workstation has the capacity to store up to 27 tip boxes in the 3 independent tip rack holders, providing plate and tip capacity for even the most complicated NGS library method. The Sciclone® G3 NGSx iQ™ workstation has a unique plate and tip sensor enabling its robotic arm to sense the presence of an object. Multiple tip types can be housed to accommodate various liquid transfer requirements. Highly accurate, low volume dispensing can be achieved with the 35 µl filter tips and 80 and 150 µl tips are available for washes and transfers.

Applications

• Methods automated on the Sciclone G3 NGSx iQ workstation

• Complete NGS workflows

Specifications

AUTOMATED NGS WORKFLOW

Sciclone® G3 NGSx iQ™ Workstation-Deck Overview

Confidence in the Generation of High-quality NGS Libraries

The Sciclone® G3 NGSx iQ™ workstation offers precise liquid transfers enhancing experimental reproducibility.

Take the Next Step in Automation with an On-deck Thermal Cycler

PerkinElmer has incorporated an on-deck thermal cycler (ODTC) to ensure full walk away automation for those who wish to have thermal cycling capability on deck. The ODTC enables numerous capabilities in library prep including:

• Automated cDNA synthesis in RNA-seq workflows

• PCR

• Steps such as fragmentation which require high temperatures

• Adenylation & end repair

A Proven History of High-Quality NGS Library Construction

PerkinElmer offers an extensive library of standardized and tested NGS protocols which automate library prep kits from PerkinElmer, Illumina, Twist, NEB, Roche, and many other companies on our liquid handlers. These automated methods must pass our stringent quality control metrics and those of collaborators we work with. Automated protocols currently available on the Sciclone® G3 NGSx iQ™ workstation include:

• PerkinElmer® NEXTFLEX® Rapid XP DNA-Seq Kit

• PerkinElmer® NEXTFLEX® Small RNA-Seq Kit v3

• Illumina® Nextera® XT DNA Kit

• Illumina® Nextera® Flex DNA Kit

• Illumina® TruSeq® Stranded mRNA Library Prep Kit

• Agilent® SureSelect® XT Kit

• Roche® KAPA® DNA HyperPrep™ Kit

Eliminate Library Set-up Time

Pre-plated NEXTFLEX® library prep kits eliminate the need for initial run set-up, offering plug-and-play library construction on the Sciclone® G3 NGSx iQ™ workstation. These pre-plated kits reduce total library prep time, hands-on time, human error, and loss of efficiency due to reagent dead volume.

PKeye™ Mobile Operations Monitor

PerkinElmer’s PKeye™ mobile operations monitor implements multiple on-deck cameras to provide:

• Remote error notification

• Access to recorded videos to review past errors

• Locally stored full run footage for future troubleshooting

Lids, Plates & Reservoirs:

• Universal Lid

• PCR Plate

• On-Deck Thermal Cycler Disposable Lids

• Polypropylene Low-Volume Microplate

• Polypropylene Storage Block

• Polypropylene Deep-Well Storage Plate

• Polypropylene Storage Plate

• Polypropylene Deep-Well Reservoir

Pipette Tips:

• 80 μL

• 100 μL

• 150 μL

• 200 μL