The Sciclone® G3 NGSx HT workstation coupled with a new NEXTFLEX® Rapid XP DNA-seq kit takes the guesswork out of the miniaturization in NGS library prep. This unique library preparation workflow enables researchers to increase their NGS throughput without increasing their budget while dramatically decreasing their cost per reaction. While other companies have miniaturized single-steps within the NGS library preparation workflow, this is the only full, standardized solution for complete NGS library prep reaction miniaturization.

Features

• Complete miniaturization solution including instrumentation and reagents.

• Fully automated – set it up and walk away.

• Standardized and tested miniaturized protocols.

• Library prep reagents optimized and tested for low volume library construction.

• Ultra-high-throughput multiplexing capabilities with 1,536 UDI barcodes.

• Only vendor supporting complete automated library prep miniaturization solutions.

Fully Automated, Walk-Away Miniaturized Workflows

The Sciclone® G3 NGSx HT workstation offers precision pipetting down to 500 nL, allowing for the transfer of enzymes, samples, beads, and buffers at volumes sufficient to scale reactions down during NGS library preparation. The NGS workstation comes equipped with a 384-low volume pipetting head, on-deck thermal elements, shaker, thermal cycler, and robotic pipette tip loader for truly walkaway sample preparation of high-quality NGS libraries from purified nucleic acids or single cell inputs. The workstation is well positioned to automate:

• cDNA Synthesis from RNA or single cells

• Library construction from cDNA or gDNA

The Sciclone® G3 NGSx HT workstation includes the following components:

1. Robotic Tip Loader with a capacity of 27 384-tip boxes

2. InHeco® On-deck Thermal Cycler for automation of PCR and multi-temperature incubations

3. InHeco® CPAC for heating and chilling of samples and reagents

4. InHeco® Thermal Shaker for thermally controlled shaking and mixing during incubations

5. Sciclone® Low-volume Pipette head for precise liquid transfers down to 500 nL

6. Integrated gripper for moving plates, lids, and tip boxes around the deck or to waste

7. Through-deck tip chute for disposal of spent pipette tips

Library Prep Reagents Optimized and Validated for Reaction Miniaturization

High quality DNA-seq libraries can be prepared using the NEXTFLEX® Rapid XP DNA-seq kit for the Sciclone® G3 NGSx HT workstation, specially designed for reaction miniaturization on this instrument. The NEXTFLEX® Rapid XP DNA-seq kit is based on a streamlined protocol incorporating a single step for fragmentation, end-repair, and adenylation with minimal hands-on time to generate NGS libraries with consistent yields and ultimately produce high-quality sequencing metrics. All the required reagents for fragmentation, library prep, and magnetic bead-based cleanup are supplied with the kit. For ultra-high-throughput multiplexing, up to 1,536 NEXTFLEX® Unique Dual Index barcodes are now available off-the-shelf.

PKeye™ Mobile Operations Monitor

The PKeye™ mobile operations monitor incorporates on-deck cameras with the Sciclone® G3 NGSx HT workstation. Through the PKeye™ cloud-based platform, you can monitor your workstation from a conference room, office, or your own home. This system will notify you of any errors that occur, allowing you to walk away from the workstation and focus your attention on intellectual contributions to the lab.

Specifications

Lids, Plates & Reservoirs:

• Universal Lid

• Polypropylene Deep-well plate

• HardShell PCR Plate

• On-deck Thermal Cycler Disposable Lids

Pipette Tips:

• 25 µL