Next generation sequencing (NGS) technologies are enabling science at unprecedented rates. As the cost of sequencing declines, throughput continues to increase and the number of applications for which sequencing data can be used is expanding. The Sciclone® G3 NGSx liquid handling workstations is designed for high throughput, walkaway NGS library preparation. The deck design and intuitive interface virtually eliminate cross contamination and user error. This liquid handlers simplify the construction of up to or greater than 96 libraries per day.

With over 88 automated library prep, sequence capture, and normalization protocols currently developed for use with the Sciclone G3 NGSx workstation, the time and effort associated with protocol automation is greatly reduced. Automated library prep protocols have been developed for products from:

• PerkinElmer

• Illumina

• Agilent

• New England BioLabs

• 10X Genomics

• Roche

• Swift Biosciences

• Nugen

• Pacific Biosystems

• Thermo Fisher Scientific

PerkinElmer has developed the PKeye™ mobile operations monitor which facilitates true walk-away automation through integrated on-deck cameras. Uploading any workstation to the Pkeye™ cloud-based platform, this system allows you to have true worry-free walk-away automation of even the most complex library preparation workflows.  The PKeye™ mobile operations monitor sends you a text message when your in-process libraries are ready to be moved to a thermal cycler for amplification allowing you to truly walkaway from your Sciclone® G3 liquid handler and focus on intellectual contributions in the lab.

Applications

• Methods automated on the Sciclone G3 NGS workstation

• Complete NGS workflows

Specifications

Lids, Plates & Reservoirs:

• Universal Lid

• PCR Plate

• Polypropylene Low-Volume Microplate

• Polypropylene Storage Block

• Polypropylene Deep-Well Storage Plate

• Polypropylene Storage Plate

• Polypropylene Deep-Well Reservoir

Pipette Tips:

• 80 μL

• 100 μL

• 150 μL

• 200 μL

Publications that Cite Using the Sciclone® G3 NGS Workstation

Brandstetter, B., Dalwigk, K., Platzer, A., Niederreiter, B., Kartnig, F., Fischer, A., . . . Karonitsch, T. (2019). FOXO3 is involved in the tumor necrosis factor-driven inflammatory response in fibroblast-like synoviocytes. Laboratory Investigation. doi:10.1038/s41374-018-0184-7

Corominas, J., Colijn, J. M., Geerlings, M. J., Pauper, M., Bakker, B., Amin, N., . . . Hollander, A. I. (2018). Whole-Exome Sequencing in Age-Related Macular Degeneration Identifies Rare Variants in COL8A1 , a Component of Bruch’s Membrane. Ophthalmology. doi:10.1016/j.ophtha.2018.03.040.

Faiz, A., Heijink, I. H., Vermeulen, C. J., Guryev, V., Berge, M. V., Nawijn, M. C., & Pouwels, S. D. (2018). Cigarette smoke exposure decreases CFLAR expression in the bronchial epithelium, augmenting susceptibility for lung epithelial cell death and DAMP release. Scientific Reports,8(1). doi:10.1038/s41598-018-30602-7.

Griss, J., Bauer, W., Wagner, C., Maurer-Granofszky, M., Simon, M., Chen, M., . . . Wagner, S. N. (2018). B cells sustain inflammation and improve survival in human melanoma:. doi:10.1101/478735

Gösch, L., et al. (2018) A T cell-specific deletion of HDAC1 protects against experimental autoimmune encephalomyelitis. Journal of Autoimmunity. (86). doi.org/10.1016/j.jaut.2017.09.008.

Lange, M., Siemann, S., Woestmann, C., Mcnamara, S., Feldkamp, M., Blueher, A., . . . Rosenthal, A. (2018). Abstract 1413: Automation of a barcoded ctDNA sequencing assay based on hybrid capture on two robotic pipetting platforms enables processing of large numbers of liquid biopsy samples. Cancer Research,78(13 Supplement), 1413-1413. doi:10.1158/1538-7445.am2018-1413.

Li, Jin, et al. (2017) Artemisinins Target GABAA Receptor Signaling and Impair a Cell Identity. Cell. 168 (1) 86 – 100.e15.

Ong, J., Woldhuis, R. R., Boudewijn, I. M., Berg, A. V., Kluiver, J., Kok, K., . . . Brandsma, C. A. (2019). Age-related gene and miRNA expression changes in airways of healthy individuals. Scientific Reports,9(1). doi:10.1038/s41598-019-39873-0.

Rosenberg, A. Z., Wright, C., Fox-Talbot, K., Rajpurohit, A., Williams, C., Porter, C., . . . Halushka, M. K. (2018). XMD-miRNA-seq to generate near in vivo miRNA expression estimates in colon epithelial cells. doi:10.1101/333658.

Oxnard, G. et al. (2020) Adjuvant Lung Cancer Enrichment Marker Identification and Sequencing Trial (ALCHEMIST). Downloaded from https://mtcancer.org/wp-content/reagent/Uploads/2020/02/A151216-07.pdf.

Wong, T. N., Miller, C. A., Jotte, M. R., Bagegni, N., Baty, J. D., Schmidt, A. P., . . . Link, D. C. (2018). Cellular stressors contribute to the expansion of hematopoietic clones of varying leukemic potential. Nature Communications, 9(1). doi:10.1038/s41467-018-02858-0.