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NEXTFLEX® RNA-Seq Barcodes

Up to 48 unique adapters available

Validated on Illumina® next-generation sequencing platforms

Considerably reduce your per-sample sequencing cost by multiplexing libraries

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  • 试剂组分
  • 引用文献

Added Flexibility to Library Preparation

The NEXTFLEX® RNA-Seq Barcodes are indexed adapters that are designed for use with the NEXTFLEX® Rapid RNA-Seq Kit and the NEXTFLEX® Rapid Directional RNA-Seq Kit. These barcodes are not recommended for use with the new NEXTFLEX® Rapid Directional RNA-seq kit 2.0.


These indexes are composed of a 6 nt error-correcting sequence, offering an improved multiplex workflow and adding flexibility to library prep. The ability to pool samples in an efficient way significantly decreases hands on time while providing robust data quality. Their automation-friendly format enables multiplexing of up to 48 samples. The NEXTFLEX® RNA-Seq Barcodes are available in sets of 6, 12, 24 and 48 unique adapters. We also offer the NEXTFLEX-96™ RNA-Seq Barcode Kits, which contain 96 – 8 nt index barcodes in a 96-well format (cat # NOVA-512915) .


Avoiding Registration Failure with Low Level Multiplexing

Registration failure could occur if the color balance was not maintained between the red and green lasers (used to sequence A/C bases and G/T bases, respectively). Read our blog post, Tech Tips – Barcode Recommendations for Low Level Multiplexing, to learn how to avoid registration failure on an  Illumina® sequencer caused by lack of sufficient index sequence diversity.


产品特点:

  • Indexed adapters compatible with Illumina® platforms for multiplexing libraries

  • Up to 48 unique adapters available

  • Considerably reduce your per-sample sequencing cost by multiplexing libraries

  • Increase your sequencing scale by pooling 100s of samples on a single flow cell

  • Validated on Illumina® next-generation sequencing platforms

  • Compatible with the NEXTFLEX® Rapid RNA-Seq Kit and the NEXTFLEX® Rapid Directional RNA-Seq Kit

  • Not compatible with the NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0


产品列表:

货号产品名称 规格
NOVA-512911NEXTFLEX® RNA-Seq Barcodes-648 RXNS
NOVA-512912 NEXTFLEX® RNA-Seq Barcodes-1296 RXNS
NOVA-512913NEXTFLEX® RNA-Seq Barcodes-24192 RXNS
NOVA-512914NEXTFLEX® RNA-Seq Barcodes-48384 RXNS
NOVA-512915

NEXTFLEX® RNA-Seq Barcodes-96(in 96-well plate)

768 RXNS


KIT CONTENTS


  • NEXTFLEX® RNA-Seq Barcode Adapter (0.6 µM)

  • NEXTFLEX® Primer Mix (12.5 µM)


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