DNA and RNA quantitation and sizing can be done in seconds using automated capillary electrophoresis separation. The LabChip® GX Touch™ nucleic acid analyzer’s microfluidic technology generates reproducible, high-resolution data and is optimal for:

• Fast, high-throughput QC analysis of NGS libraries for detection of mutations associated with SARS-CoV-2

• NGS library preparation (smear and fragment analysis) and quality control

• RNA and DNA fragment analysis (including cell-free DNA, DNA isolated from FFPE samples, and PCR-free libraries)

• Quantitation and qualification for CRISPR fragment analysis

• Screening nucleic acid source material for SARS-CoV-2 vaccine development

• Characterization and QC of small RNA molecules and CRISPR/Cas9 gRNA

• Multiplex Detection of SARS-CoV-2 Multiplex Detection of SARS-CoV-2 Variants of Concern using ARMS-PCR

The LabChip GX Touch nucleic acid analyzer also supports a variety of DNA and RNA analysis, which provides unparallel genomic data quality with minimal input requirements. When the concentrations are low, and samples are precious, The LabChip® DNA High Sens and RNA Pico assays are ideal choice for reliable measurement and sample preservation. With complete analysis of genomic material in about 30 seconds, the LabChip GX Touch nucleic acid analyzer eliminates the nucleic acid quantitation workflow bottleneck. High-resolution analysis provides exact sizing and quantitation of DNA fragments and smears down to 5 bp from samples as small as 2 pg/µL.

The system offers a broad variety of DNA analysis options, including the NGS 3K assay, which provides unparalleled genomic data quality with minimal input requirements. This high degree of sensitivity also makes it the ideal instrument for gDNA, cfDNA, and PCR-free DNA quantitation, where concentrations are low, samples are precious and their preservation is essential.

Purity and Integrity Analysis of mRNA-based Vaccines

By using LabChip® RNA Assay, researchers at Merck developed an assay to characterize purity and integrity of mRNA vaccines. This fast, easy and efficient workflow helped speed up vaccine development and meet increased manufacturing high volume demand.

Features

The Comprehensive Solution for Genomic Sizing & Quantitation

• Quantitate DNA and RNA sample integrity hours faster than agarose gels

• Maximize rare or low-concentration samples like cfDNA, PCR-free libraries, and ChIP-seq libraries

• Accurate quantitation down to 25 pg/µL for DNA smears and 0.5 pg/µL for fragments

• NGS library prep analysis and QC

• Flexible throughput options make it cost-efficient – 96-well or 384-well (HT) format

• Data visualization options (electropherogram, virtual gel, or tabular report)

Applications

• Complete NGS workflows

• CRISPR Fragment Analysis

• Vaccine Development

Specifications

Windows 10-compatible software

Increasing Speed and Throughput of SARS-CoV-2 Variant Detection Workflows

The capacity and speed of analysis make the LabChip® NGS 3K assay run on the LabChip® GX Touch™ nucleic acid analyzer an ideal component of high-throughput NGS-based variant detection workflows. Figure 1 illustrates examples of fully constructed library of CoV+ sample with pooled amplicon input at 200 ng (blue line) and 1200 ng (red line). It only takes a minute to examine NEXTFLEX® Variant Seq™ SARS-CoV-2 libraries, to ensure proper library sizing quality and exclusion of contaminating fragments.

• Minimal sample consumption to save constructed library for next sequencing steps

• Automated process for high measurement reproducibility and operation reliability

• Multiple digitized data formats for convenient data analysis, visualization, and sharing

Figure 1: Validation of NEXTFLEX® Variant Seq™ SARS-CoV-2 Libraries.

Libraries of CoV+ samples were analyzed using the LabChip® NGS 3K assay on the LabChip® GX Touch™ nucleic acid analyzer: 200 ng pooled amplicon input (blue) and 1200 ng pooled amplicon input (red).

The system offers a broad variety of DNA analysis options, including the NGS 3K assay, which provides unparalleled genomic data quality with minimal input requirements. This high degree of sensitivity also makes it the ideal instrument for gDNA, cfDNA, and PCR-free DNA quantitation, where concentrations are low, samples are precious, and their preservation is essential.

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