Prevent Poor Reads Caused by Single Base Errors

The NEXTFLEX® DNA barcodes for Ion Torrent™ sequencing platforms can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTFLEX® DNA barcodes use an indexed adapter with a 10 nt unique sequence, allowing for proper differentiation between samples and preventing poor reads caused by single base errors introduced during PCR. Up to 64 samples can be multiplexed using these adapters. The NEXTFLEX® DNA barcodes undergo stringent quality controls and are functionally validated by sequencing.

These barcodes are compatible with the NEXTFLEX® DNA library prep kits for Ion Torrent™ sequencers, the NEXTFLEX® Cell Free DNA-seq kit for Ion Torrent™ sequencers, and other genomic and ChIP-seq DNA library prep protocols compatible with the Ion Torrent™ PGM™, Ion Proton™, Ion S5™, and Ion S5™ XL sequencers.

Features

• Up to 64 samples can be multiplexed

• Compatible with DNA-Seq and ChIP-Seq library prep on the Thermo Fisher® Scientific Ion S5™, Ion S5™ XL, Ion PGM™, and Ion Proton™ sequencing platforms

• Considerably reduce your per-sample sequencing cost using barcoded multiplexing

• Increase your sequencing scale by pooling 100s of samples on a single flow cell

Kit Specs

KIT CONTENTS

• NEXTFLEX® DNA P1 Adapter (6.25 µM)

• NEXTFLEX® DNA Barcode Adapters (6.25 µM)

• NEXTFLEX® Primer Mix (12.5 µM)

Selected Publications that Reference Using the NEXTFLEX® DNA Barcodes for Ion Torrent™ Multiplexing:

Appenzeller, S. et al. (2015) Immunoglobulin rearrangement analysis from multiple lesions in the same patient using Next Generation Sequencing. Histopathology. doi: 10.1111/his.12714.

Argueta, W. C. (2015) Comparison of Next Generation Sequencing Methodology on the Ion PGM System Performance versus that on the Sanger Sequencing Method for HV1 and HV2 Regions of mtDNA. University of North Texas Health Science Center. UNTHSC Scholarly Repository.

Lundberg, P. et al. (2014) Clonal evolution and clinical correlates of somatic mutations in myeloproliferative neoplasms. Blood 123(14): 2220-2228.

Raphael, B. H., Shirey, T. B., Lúquez, C. and Maslanka, S. E. (2014) Distinguishing highly-related outbreak-associated Clostridium botulinum type A(B) strains. BMC Microbiol. 192: 14.