Whole Genome Enrichment (WGE) is target capture of genome-wide DNA from a complex DNA source.

Daicel Arbor Biosciences’WGE bait manufacturing technology can cost-effectively produce biotinylated RNA baits representative of an entire nuclear genome. This allows for bulk enrichment of genome-wide endogenous DNA from complex metagenomic samples, such as environmental or ancient DNA. To manufacture custom WGE baits, all that is required is a sample of high-quality genomic DNA from an organism of interest, or a close relative, even if the genome has not been sequenced.

Recovering appreciable full-genome coverage from metagenomic, environmental, or ancient DNA samples with only shotgun sequencing data is likely to be both expensive and bioinformatically intense. Standard and custom WGE kits from Daicel Arbor Biosciences can help overcome these challenges. Discover how myBaits WGE kits can dramatically increase the percentage of endogenous DNA reads from an NGS dataset.

Due to the high sequencing depth required to observe appreciable coverage of whole-genome data, especially from degraded or complex samples, Whole Genome Enrichment (WGE) kits are generally best suited for exploratory research projects. If the goal is to recover specific genes or markers of interest, then a focused custom myBaits panel is more appropriate for maximizing sequencing efficiency. If WGE is the proper application of choice, premade and custom WGE kits from Daicel Arbor Biosciences can help overcome many of these challenges.

• Custom bait sets – Select from an organism of interest

• Premade human baits – In stock and ready to ship for ancient DNA

• Efficient genome-wide sequencing – Isolate genome of interest from complex samples

• Cost-effective genomic bait manufacturing – Savings on baits and sequencing costs

• Perfect for exploratory studies – Sequenced genome is not required

Kit Specs

TIMELINE

Human WGE kits are in-stock for immediate shipment. Custom WGE kits typically require 2-4 weeks for production (starting from when Arbor receives a suitable genomic DNA sample)

WHAT'S INCLUDED?

Supplied as complete kit, including probes as well as all necessary reagents for the hybridization capture and wash steps.

Ordering Process

Daicel Arbor Biosciences has in-stock genomic DNA (gDNA) WGE panels for Human (Caucasian, African, or Japanese Male). To produce a Custom WGE panel, a high-purity, high-molecular weight gDNA sample is required to be shipped to Arbor’s manufacturing facility in Ann Arbor, MI, USA. You can ship this gDNA sample (lab-generated or commercially-sourced) to Arbor for processing, or for some organisms Arbor may be able to provide the additional service of acquiring the gDNA from a third-party vendor.Daicel Arbor Biosciences will then utilize our proprietary technology to transform the gDNA into ready-to-use genome-wide target capture baits.

Workflow

Figure 1. Schematic demonstrating the production and typical application of myBaits WGE kits.

Baits can be produced from any pure eukaryotic or prokaryotic genomic DNA source, for example modern elephant. Daicel Arbor Biosciences’ unique process coverts this gDNA into a pool of biotinylated RNA baits. These baits can then enrich corresponding molecules from a user-supplied NGS library, for example from an ancient mammoth, via the process of myBaits in-solution hybridization capture.

This enriched library allows for orders-of-magnitude more efficient NGS than would be possible using the full, non-enriched library.

Featured Publications

H. McColl et al. (2018). The prehistoric peopling of Southeast Asia. Science.

J. Lindo et al. (2017). Ancient individuals from the North American Northwest Coast reveal 10,000 years of regional genetic continuity. PNAS.

L. Vezzulli et al. (2017). Whole-Genome Enrichment Provides Deep Insights into Vibrio cholerae Metagenome from an African River. Microbial Ecology.

J.M. Enk et al. (2014). Ancient Whole Genome Enrichment Using Baits Built from Modern DNA. Molecular Biology and Evolution.