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NEXTFLEX® DNA-Seq Kits for Ion Torrent™ Sequencers

较高的接头连接效率,产出大量不同的reads

灵活的接头方案供选择—试剂盒包含8、16、32和64重独特barcodes

简化实验流程,实现自动化建库

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  • 产品简介
  • 工作流程
  • 试剂组分
  • 引用文献

NEXTflex™ DNA Sequencing Kit - Ion Torrent Compatible 适用于 genomic DNA-Seq或 ChIP-Seq高通量测序建库,兼容于Ion S5, Ion S5 XL,Ion Torrent PGM 和 Ion Proton 测序平台。NEXTflex DNA Sequencing Kit 采用了“Enhanced Adapter Ligation Technology ”,得到更长的、种类更多的测序reads。这款 DNA-Seq library prep kit 通过使用酶预混液、凝胶纯化来简化文库制备流程。此外,可在96孔板中进行建库反应,可更好的实现建库自动化。


兼容于Ion S5, Ion PGM 和 Ion Proton 平台的多重DNA建库

NEXTflex™ DNA Barcodes - Ion Torrent Compatible中接头包含index序列,改进了多重建库实验流程,样本起始量也可进行灵活的选择。可同时进行多达64种样本建库。NEXTflex DNA Barcodes分别包含8, 16,32, and64重独特接头。此建库方案可有效的减少实验耗时,获得稳定的实验数据。


产品特点:

  • 较高的接头连接效率,产出大量不同的reads

  • 灵活的接头方案供选择—试剂盒包含8、16、32和64重独特barcodes

  • 简化实验流程,实现自动化建库

  • 适用于 Ion Torrent 平台的ChIP-seq 和DNA-seq 建库

  • 起始量为10 ng,100 ng或 1 μg 基因组DNA


产品列表:

货号产品名称规格
NOVA-4001-01

NEXTFLEX® DNA-Seq Kits for Ion Torrent™ Sequencers

8 RXNS
NOVA-4001-02

NEXTFLEX® DNA-Seq Kits for Ion Torrent™ Sequencers

48 RXNS
NOVA-401001

NEXTflex™ DNA Barcodes-Ion Torrent Compatible - 8

48 RXNS
NOVA-401002

NEXTflex™ DNA Barcodes-Ion Torrent Compatible - 16

96 RXNS
NOVA-401003

NEXTflex™ DNA Barcodes-Ion Torrent Compatible - 32

192 RXNS
NOVA-401004

NEXTflex™ DNA Barcodes-Ion Torrent Compatible - 64

384 RXNS


NEXTflex™ DNA-Seq Kit Protocol

KIT CONTENTS


  • NEXTFLEX® End Repair Buffer Mix

  • NEXTFLEX® End Repair Enzyme Mix

  • NEXTFLEX® Ligation Mix

  • NEXTFLEX® Adapter Mix (6.25 µM)

  • NEXTFLEX® Primer Mix (12.5 µM)

  • NEXTFLEX® 5X PCR Master Mix

  • 6X Loading Dye

  • MW Ladder Ready-to-Load 100 bp

  • Column Elution Buffer

  • Nuclease-free Water

  • Resuspension Buffer

  • DNA Binding Buffer

  • 5X DNA Wash Buffer

  • Clean-Up Spin Columns


REQUIRED MATERIALS NOT PROVIDED


  • 10 ng, 100 ng or 1 µg of fragmented genomic DNA in up to 40 µL nuclease-free water.

  • NEXTFLEX® DNA Barcodes Ion Torrent Compatible – 8 / 16 / 32 / 64 (Cat # 4010-01, 4010-02, 4010-03, 4010-04)

  • Ethanol 100% (room temperature)

  • Ethanol 80% (room temperature)

  • 96 well PCR Plate Non-skirted (Phenix Research, Cat # MPS-499) / or / similar

  • 96 well Library Storage and Pooling Plate (Fisher Scientific, Cat # AB-0765) / or / similar

  • Adhesive PCR Plate Seal (BioRad, Cat # MSB1001)

  • Agencourt AMPure XP 5 mL (Beckman Coulter Genomics, Cat # A63880)

  • Magnetic Stand -96 (Ambion, Cat # AM10027) / or / similar

  • Heat block

  • Thermocycler

  • 2, 10, 20, 200 and 1000 mL pipettes / multichannel pipettes

  • Nuclease-free barrier pipette tips

  • Microcentrifuge

  • 1.5 mL nuclease-free microcentrifuge tubes

  • Low melt agarose such as Low Gelling Temperature Agarose with a melt point of 65ºC (Boston Bioproducts, Cat # P-730) / or / E-gel system

  • 1X TAE buffer

  • Clean razor or scalpel

  • SYBR Gold (Invitrogen, Cat # S11494)

  • UV transilluminator or gel documentation instrument

  • Gel electrophoresis apparatus

  • Electrophoresis power supply

  • Vortex


NEXTflex DNA Sequencing Kit for Ion Torrent Sequencing部分引用文献

Appenzeller, S. et al. (2015) Immunoglobulin rearrangement analysis from multiple lesions in the same patient using Next Generation Sequencing. Histopathology. doi: 10.1111/his.12714.

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