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NextPrep-Mag™ Urine cfDNA Isolation Kit

优化尿液中DNA提取方案

以很少的PCR 循环数即可得到高浓度文库

30min完成整个提取过程,操作简便

无需真空泵、真空歧管、填充柱

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  • 产品简介
  • 工作流程
  • 试剂组分
  • 引用文献

NextPrep-Mag™ Urine cfDNA Isolation Kit

NextPrep-Mag™ Urine cfDNA Isolation Kit 运用磁珠法提取人类尿液cfDNA,用于NGS建库,操作快速简便。本产品提取产物可用于二代测序和PCR 分析。符合要求的DNA可被用于NGS建库,且已经过Bioo Scientific 的NEXTflex™ Cell Free DNA-Seq Library Prep Kit for Illumina 和NEXTflex Cell Free DNA Library Prep Kit for Ion Proton、Ion PGM 验证。


产品特点:

  • 优化尿液中DNA提取方案

  • 以很少的PCR 循环数即可得到高浓度文库

  • 采用磁珠法,更容易实现自动化

  • 30min完成整个提取过程,操作简便

  • 提取产物可作为NGS样本

  • 无需真空泵、真空歧管、填充柱


产品列表

货号产品名称规格
NOVA-5150-01NextPrep-Mag™ Urine cfDNA Isolation Kit for Illumina8 RXNS
NOVA-5150-02NextPrep-Mag™ Urine cfDNA Isolation Kit for Illumina48 RXNS
NOVA-4002-01NextPrep-Mag™ Urine cfDNA Isolation Kit for Ion Torrent8 RXNS
NOVA-4002-02NextPrep-Mag™ Urine cfDNA Isolation Kit for Ion Torrent48 RXNS


Cell Free DNA Isolation Protocol

整个实验流程可在大约30min 内完成,包括蛋白酶解、DNA结合、快速洗涤、cfDNA 洗脱。

KIT CONTENTS


  • cfDNA DNA Proteinase K

  • Urine cfDNA Binding Solution

  • cfDNA Magnetic beads

  • cfDNA Wash Solution 1

  • cfDNA Wash Solution 2

  • cfDNA Elution Solution

  • Control PCR Primers


REQUIRED MATERIALS NOT PROVIDED


  • Ethanol, 100%

  • Vortex mixer

  • Heat block at 55°C

  • Magnetic stands to hold 2 mL microcentrifuge tubes and 15 mL or 50 mL tubes

  • Microcentrifuge or low-speed “picofuge”

  • Nuclease-free 2 mL (1.5 mL and 0.5 mL optional) microcentrifuge tubes and 15 mL tubes

  • Pipettors (P-1000, P-200, P-20) and tips

  • Disposable pipettes (variable sizes depending on sample volume processed)


Selected Citations that Reference the Use of the NextPrep-Mag Urine cfDNA Isolation kit:

Streleckiene, G., Reid, H. M., Arnold, N., Bauerschlag, D., & Forster, M. (2018). Quantifying cell free DNA in urine: Comparison between commercial kits, impact of gender and inter-individual variation. BioTechniques,64(5), 225-230. doi:10.2144/btn-2018-0003

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