Ideal for extracting DNA from urine for cell-free DNA analysis
Generates highly concentrated libraries using few PCR cycles
Automation friendly, mag-bead based format
Fast, 30 minute protocol with minimal hands-on time
Ideal for NGS library prep
No vacuum pumps, vacuum manifolds, or column extenders required
The NextPrep-Mag™ Urine cfDNA Isolation Kit is designed for extracting cell-free DNA (cfDNA) for constructing NGS libraries from urine, using a fast magnetic bead-based format requiring minimal hands-on time. The isolated cell free DNA is ideal for next generation sequencing and PCR analysis. Suitability of the DNA for use in NGS library construction has been verified using the Bioo Scientific NEXTflex™ Cell Free DNA-Seq Library Prep Kit for Illumina Sequencing and the NEXTflex Cell Free DNA Library Prep Kit for Ion Proton and Ion PGM Sequencing.
The NextPrep-Mag Urine cfDNA Isolation Kit For < 1 mL - 4 mL contains sufficient reagents for extraction of DNA from 50 samples of 1 mL urine or less, or 12 samples of 4 mL of urine. The NextPrep-Mag Urine cfDNA Isolation Kit For 4 mL - > 20 mL contains sufficient reagents for extraction of DNA from 25 samples of 4 mL urine or less, or 5 samples of 20 mL of urine. The kit components are stable for at least 12 months from time of receipt, when properly stored.
|NOVA-3826-01||NEXTprep-Mag™ Urine cfDNA Isolation Kit ( < 1 mL - 4 mL)||12 to 50 isolations|
|NOVA-3826-02||NEXTprep-Mag™ Urine cfDNA Isolation Kit (4 mL - 20 mL)||5 to 25 isolations|
cfDNA DNA Proteinase K
Urine cfDNA Binding Solution
cfDNA Magnetic beads
cfDNA Wash Solution 1
cfDNA Wash Solution 2
cfDNA Elution Solution
Control PCR Primers
Heat block at 55°C
Magnetic stands to hold 2 mL microcentrifuge tubes and 15 mL or 50 mL tubes
Microcentrifuge or low-speed “picofuge”
Nuclease-free 2 mL (1.5 mL and 0.5 mL optional) microcentrifuge tubes and 15 mL tubes
Pipettors (P-1000, P-200, P-20) and tips
Disposable pipettes (variable sizes depending on sample volume processed)
The procedure, which can be completed in approximately 30 minutes, includes an initial lysis and DNA binding step, wash steps, and elution of cfDNA from the magnetic beads. A distinguishing feature of the kit is the very rapid magnetic bead attraction steps.