Ideal for extracting cfDNA from up to 5 mL human plasma and serum for cell-free DNA sequencing and other downstream applications
Automation friendly, magnetic bead-based format without compromising purity or yields
Supported on the chemagic™ 360 and chemagic™ Prime™ instruments
Fast, 15 – 30 minute hands-on-time and approximately 1.5 hour turnaround for 24 samples using a 24-rod head or 96 samples using a 96-rod head
Complete Solution for Isolation of Circulating DNA from Plasma for Downstream Applications
The NextPrep-Mag™ cfDNA Automated Isolation Kit is a complete solution for automated cell-free DNA extraction from human blood plasma or serum using the chemagic™ 360, chemagic™Prime™ or chemagic™MSM I instruments from PerkinElmer.
The challenges of extraction of cfDNA from plasma or serum are several, but are rooted in the fact that the circulating DNA is highly fragmented and present at low concentrations, especially in healthy individuals. The NextPrep-Mag™ cfDNA Automated Isolation Kit was developed to facilitate the workflow demands for high throughput labs while delivering optimal results.
The NextPrep-Mag™ cfDNA Automated Isolation Kit allows for the extraction of cell-free DNA from human blood plasma or serum using the chemagic™ 360, chemagic™ Prime™ or chemagic™ MSM I instruments. The kit is all-inclusive and contains ready-to-use reagents and disposable plastics for 240 automated cfDNA isolations from up to 5 mL of starting plasma/serum. The cfDNA recovered can be used in a wide variety of downstream applications including next-gen sequencing and PCR analysis.
The cfDNA isolated with the NextPrep-Mag™ cfDNA kits have been verified to be compatible with both manual and automated NGS library construction on the Sciclone® G3 NGS workstation and the Zephyr® NGS workstation using the NEXTFLEX® Rapid DNA-Seq kit 2.0 for Illumina® sequencing.
|NOVA-3825-05||NEXTprep-Mag™ cfDNA Isolation Kit (5 mL)||240 isolations|
|NOVA-3825-06||NEXTprep-Mag™ cfDNA Isolation Kit (5 mL)||48 isolations|
|NOVA-3825-10||NEXTprep-Mag™ cfDNA Isolation Kit (1.5 mL)||960 isolations|
cfDNA Lysis/Binding Solution
cfDNA Proteinase K
cfDNA Magnetic Beads
cfDNA Wash 1 Solution
cfDNA Wash 2 Solution
cfDNA Elution Solution (10 mM Tris/0.1 mM EDTA, pH 8)
chemagic™ 360 PLATFORM PLASTICS
Rod-head specific plates, tubes, and elution tubes/plates
Please refer to respective protocol guides for details on plastics
Distilled deionized water used to dissolve Proteinase K
5 mL disposable pipets
The kit generated robust cfDNA recovery from both plasma and serum on the chemagic™ 360 and chemagic™ MSM I platforms as analyzed with electropherogram traces and Qubit® spectrophotometer.
Figure 1: Performance on the chemagic™ 360 Instrument. cfDNA was extracted from plasma (PL) or serum (SER) from 5 donors. (A) Yields were quantified electropherogram traces (for mononucleosome peak) and Qubit® spectrophotometer (for total DNA yield). (B) Overlay traces of triplicate 4 mL extractions.
Figure 2: The NextPrep-Mag™ cfDNA Automated Isolation Kit is robust on the chemagic™ MSM I instrument. Blood from different donors was collected in either (A) SST tubes or (B) Streck BCT tubes and extracted for serum. Serum was pooled from each collection method and 5 mLs were extracted using the automated protocol.
Figure 3: Sequencing results from cfDNA extracted using NextPrep-Mag™ chemistry shows good concordance with expected mutation frequencies (A, B).