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DNA快速建库试剂盒 NEXTflex® Rapid DNA-Seq Kit

DNA建库试剂盒 NEXTflex® Rapid DNA-Seq Kit—兼容Illumina测序平台



Bioo Scientific 公司的NEXTflex® Rapid DNA-Seq Kit兼容于 Illumina测序平台的测序建库,样品DNA起始量可少至1 ng,整个建库流程仅用2 小时或更短的时间。此试剂盒适用于基因组DNA、福尔马林固定石蜡包埋(FFPE)样本、ChIP DNA和低起始量的临床样本的建库。NEXTflex® Rapid  DNA-Seq  Kit 样品的起始量范围非常灵活,跨越了3个数量级,从1 ng 至1 ug 。这是一个基于磁珠纯化、无需电泳进行片段选择的实验方案,多达2,380 种接头可有效运用于多重测序。NEXTflex® Rapid DNA-Seq Kit与“Enhanced Adapter Ligation Technology ”联合使用,可以有效的连接更长的接头,得到更长的、种类更多的测序reads。Bioo Scientific公司的连接酶与聚合酶预混液可以确保建库过程的高质量进行。


规格产品名称
规格
NOVA-5144-01NEXTflex® Rapid DNA Sequencing Kit8 rxns
NOVA-5144-02NEXTflex® Rapid DNA Sequencing Kit48 rxns
NOVA-5144-08NEXTflex® Rapid DNA Sequencing Kit96 rxns
NOVA-5144-03NEXTflex® Rapid DNA-Seq Kit Bundle with DNA Barcodes 1 - 2448 rxns
NOVA-5144-04NEXTflex® Rapid DNA-Seq Kit Bundle with DNA Barcodes 25 - 4848 rxns
DNA起始量少于10 ng 搭配使用的barcode
NOVA-514120NEXTflex® ChIP-seq Barcodes - 648 rxns
NOVA-514121NEXTflex® ChIP-seq Barcodes - 1296 rxns
NOVA-514122NEXTflex® ChIP-seq Barcodes - 24192 rxns
NOVA-514123NEXTflex® ChIP-seq Barcodes - 48384 rxns
NOVA-514124NEXTflex-96™ ChIP-seq Barcodes768 rxns
DNA起始量多于10 ng 搭配使用的barcode
NOVA-514101NEXTflex® DNA Barcodes - 648 rxns
NOVA-514102NEXTflex® DNA Barcodes - 1296 rxns
NOVA-514103NEXTflex® DNA Barcodes - 24192 rxns
NOVA-514104NEXTflex® DNA Barcodes - 48384 rxns
NOVA-514105NEXTflex® DNA Barcodes - 96768 rxns


DNA建库试剂盒NEXTflex® DNA-Seq Kit - 兼容Ion Torrent平台


DNA-Seq LIbrary Prep for Ion S5™, Ion S5™ XL, Ion Torrent™ PGM™ and Ion Proton™ sequencers


货号产品名称规格
NOVA-4001-01NEXTflex® DNA-Seq Kit for Ion Torrent™ sequencers8 rxns
NOVA-4001-02NEXTflex® DNA-Seq Kit for Ion Torrent™ sequencers48 rxns
NOVA-401001NEXTflex® DNA Barcodes for Ion Torrent™ sequencers - 848 rxns
NOVA-401002NEXTflex® DNA Barcodes for Ion Torrent™ sequencers - 1696 rxns
NOVA-401003NEXTflex® DNA Barcodes for Ion Torrent™ sequencers - 32192 rxns
NOVA-401004NEXTflex® DNA Barcodes for Ion Torrent™ sequencers - 64384 rxns


说明书

产品组分

实验流程

实验数据

引用文献

相关产品


试剂盒组分

NEXTflex™ End-Repair & Adenylation Buffer Mix
NEXTflex™ End-Repair & Adenylation Enzyme Mix 
NEXTflex™ Ligase Enzyme Mix
NEXTflex™ PCR Master Mix
Nuclease-free Water
NEXTflex™ Sizing Solution
NEXTflex™ Resuspension Buffer


用户自备材料

1 ng - 1 µg of fragmented DNA in up to 32 µL nuclease-free water.
NEXTflex™ DNA Barcodes – 6 / 12 / 24 / 48 (Cat # 514101, 514102, 514103, 514104) or NEXTflex-96™ DNA Barcodes (Cat # 514106)
Ethanol 100% (room temperature)
Ethanol 80% (room temperature)
96 well PCR Plate Non-skirted (Phenix Research®, Cat # MPS-499) or similar 
96 well Library Storage and Pooling Plate (Fisher Scientific®, Cat # AB-0765) or similar
Adhesive PCR Plate Seal (BioRad, Cat # MSB1001)
Agencourt® AMPure® XP 60 mL (Beckman Coulter Genomics®, Cat # A63880)
Magnetic Stand -96 (Thermo Fisher Scientific®, Cat # AM10027) / or / similar
Thermocycler 
2, 10, 20, 200 and 1000 µL pipettes / multichannel pipettes
Nuclease-free barrier pipette tips
Vortex


2 NEXTflex™ Rapid DNA-Seq kit.png


Rapid DNA-Seq数据分析.gif

NEXTflex® Rapid DNA-Seq kit (左)和竞争品牌N’s kit (右)的建库后片段大小覆盖率比较。(实验所用样品相同,每个样品进行3次重复实验,所有样品的纯化和片段选择全部使用AMPureXP beads,数据皆用Agilent 2100分析软件进行分析。)


Rapid DNA-Seq数据分析2.gif

NEXTflex® Rapid DNA-Seq kit和竞争品牌N’s kit连接率的直接比较。图中红色部分表示的未连接的产物比例;黄色,代表单端连接比例;绿色,代表双端连接比例。


Rapid DNA-Seq数据分析3.gif

NEXTflex® Rapid DNA-Seq kit和竞争品牌N’s kit建库比较。 NEXTflex建库转化率47.1%竞争品牌N’s为21.5%。

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